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Typical Testing Data/Standard Curve (for reference only)

CD300F elisa kit

Human CD300F PicoKine ELISA Kit

Gene Names
CD300LF; CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13
Reactivity
Human
There is no detectable cross-reactivity with other relevant proteins.
Synonyms
CD300F; Human CD300F PicoKine ELISA Kit; Human CD300F; CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD300f; CD300LF; CLM1; IGSF13; IREM1; NKIR; UNQ3105/PRO10111; CD300 molecule like family member f; CD300F elisa kit
Ordering
For Research Use Only!
Reactivity
Human
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Human CD300C
Sequence Length
239
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.
Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD300F elisa kit
Principle of the Assay: The Picokine Human CD300C Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD300C with a 96-well strip plate that is pre-coated with antibody specific for CD300C. The detection antibody is a biotinylated antibody specific for CD300C. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CD300C with immunogen: Expression system for standard: NSO, Immunogen sequence: G21-R183. The kit is analytically validated with ready to use reagents. To measure Human CD300C, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD300C in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD300C in the sample!!Background/Introduction: CMRF35-like molecule 6 (CLM-6) also known as CD300 antigen-like family member C (CD300c) is a protein that in humans is encoded by the CD300C gene. It is mapped to 17q25.1. The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,480 Da
NCBI Official Full Name
CMRF35-like molecule 1 isoform 2
NCBI Official Synonym Full Names
CD300 molecule like family member f
NCBI Official Symbol
CD300LF
NCBI Official Synonym Symbols
CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13
NCBI Protein Information
CMRF35-like molecule 1
UniProt Protein Name
CMRF35-like molecule 1
UniProt Gene Name
CD300LF
UniProt Synonym Gene Names
CD300F; CLM1; IGSF13; IREM1; NKIR; CLM-1; IREM-1; IgSF13

NCBI Description

This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923). Inhibits osteoclast formation. Induces macrophage cell death upon engagement ().

Research Articles on CD300F

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Product Notes

The Human There is no detectable cross-reactivity with other relevant proteins. CD300F cd300lf (Catalog #AAA1751723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751723 ELISA Kit recognizes Human There is no detectable cross-reactivity with other relevant proteins. CD300F. It is sometimes possible for the material contained within the vial of "CD300F, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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