Human Androgen receptor ELISA Kit | AR elisa kit

Human Androgen receptor ELISA Kit

Cat. Num. AAA764307

• Gene Names: AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human
• Synonyms: Androgen receptor; Human Androgen receptor ELISA Kit; AR (Androgen Receptor); AR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AQP5. No significant cross-reactivity or interference between AQP5 and analogues was observed.
• Sequence Length: 917

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AQP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AQP5 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AQP5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AQP5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AQP5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AQP5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 178882
• NCBI GeneID: 367
• Molecular Weight: 44,643 Da
• NCBI Official Full Name: androgen receptor
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• Protein Family: Allatostatin
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4
• UniProt Entry Name: ANDR_HUMAN

NCBI Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function: androgen binding; androgen receptor activity; ATPase binding; beta-catenin binding; chromatin binding; DNA binding; enzyme binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; receptor binding; transcription factor activity; transcription factor binding; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; androgen receptor signaling pathway; cell growth; cell proliferation; cell-cell signaling; gene expression; intracellular receptor-mediated signaling pathway; negative regulation of cell proliferation; negative regulation of integrin biosynthetic process; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription, DNA-dependent; prostate gland development; protein oligomerization; sex differentiation; signal transduction; small GTPase mediated signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transport

Disease: Androgen Insensitivity Syndrome; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Prostate Cancer; Spinal And Bulbar Muscular Atrophy, X-linked 1