Rabbit anti-Human NUP153 Polyclonal Antibody | anti-NUP153 antibody

NUP153 Polyclonal Antibody

Cat. Num. AAA3013161

• Gene Names: NUP153; N153; HNUP153
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: NUP153; Polyclonal Antibody; NUP153 Polyclonal Antibody; 153 kDa nucleoporin; HNUP153; N153; NU153; Nuclear pore complex protein hnup153; Nuclear pore complex protein Nup153; Nucleoporin 153kDa; Nucleoporin Nup153; Nup 153; Nup153; anti-NUP153 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NUP153 polyclonal antibody detects endogenous levels of NUP153 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1475

• Applicable Applications for anti-NUP153 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:100-1:500; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution.)

Immunocytochemistry (ICC)

(ICC staining NUP153 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-NUP153 antibody

Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup153 is a peripheral NPC component that is implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Nup153 contains a unique N-terminal region, a central domain consisting of four to five zinc fingers and a C-terminal region containing about 30 irregularly spaced FXFG repeats. Nup153 is cleaved by caspases during apoptosis. Nup153 interacts wtih TAP, which is essential for mRNA export, and associates with chromatin towards the end of anaphase, in parallel with the inner nuclear membrane protein LAP2. Nup153 is involved in NPC assembly, in anchoring NPCs within the nuclear envelope and in mediating specific nuclear import events.

NCBI and Uniprot Product Information

• NCBI GI #: 24430146
• NCBI GeneID: 9972
• NCBI Accession #: NP_005115.2
• NCBI GenBank Nucleotide #: NP_005115.2
• UniProt Accession #: P49790
• Molecular Weight: 154kDa
• NCBI Official Full Name: nuclear pore complex protein Nup153 isoform 2
• NCBI Official Synonym Full Names: nucleoporin 153
• NCBI Official Symbol: NUP153
• NCBI Official Synonym Symbols: N153; HNUP153
• NCBI Protein Information: nuclear pore complex protein Nup153
• UniProt Protein Name: Nuclear pore complex protein Nup153
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP153
• UniProt Entry Name: NU153_HUMAN

NCBI Description

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Protein type: Nucleoporin; Nuclear export

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nuclear pore; nuclear inclusion body

Molecular Function: identical protein binding; protein binding; DNA binding; structural constituent of nuclear pore; zinc ion binding; transporter activity; protein anchor; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; entry of virus into host cell; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; negative regulation of RNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport