MDHA / MDH1 recombinant protein

Recombinant Rat MDHA / MDH1 Protein (His tag)

Cat. Num. AAA2547405

• Gene Names: Mdh1; MDL1; Mdhl; Mor2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: MDHA / MDH1; Recombinant Rat MDHA / MDH1 Protein (His tag); MDL1; Mdhl; Mor2; MDHA / MDH1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 10% glycerol, pH 8.0
• Sequence Length: 334

• Application Notes: The recombinant rat MDH1 comprises 345 amino acids and predicts a molecular mass of 38 kDa. The apparent molecular mass of the rat MDH1 is approximately 39 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MDHA / MDH1 recombinant protein

Background: Malate dehydrogenases 1 (MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure. 

Description: A DNA sequence encoding the rat MDH1 (O88989) (Met 4-Ala 334) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 15100179
• NCBI GeneID: 24551
• NCBI Accession #: NP_150238.1
• NCBI GenBank Nucleotide #: NM_033235.2
• UniProt Accession #: O88989; O88585; Q6PCV2
• Molecular Weight: 36,483 Da
• NCBI Official Full Name: malate dehydrogenase, cytoplasmic isoform Mdh1
• NCBI Official Synonym Full Names: malate dehydrogenase 1
• NCBI Official Symbol: Mdh1
• NCBI Official Synonym Symbols: MDL1; Mdhl; Mor2
• NCBI Protein Information: malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal
• UniProt Protein Name: Malate dehydrogenase, cytoplasmic
• UniProt Gene Name: Mdh1
• UniProt Synonym Gene Names: Mdh

NCBI Description

This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016]

Uniprot Description

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.37; EC 1.1.1.96; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: centrosome; cytoplasm; cytosol; extracellular space; mitochondrion; myelin sheath

Molecular Function: L-malate dehydrogenase activity; malate dehydrogenase activity; NAD binding

Biological Process: malate metabolic process; NAD metabolic process; NADH metabolic process; oxaloacetate metabolic process; positive regulation of defense response to virus by host; tricarboxylic acid cycle