Human Hepatic triacylglycerol lipase ELISA Kit | LIPC elisa kit

Human Hepatic triacylglycerol lipase ELISA Kit

Cat. Num. AAA762785

• Gene Names: Lipc; HL; Hpl; AI256194
• Reactivity: Human
• Synonyms: Hepatic triacylglycerol lipase; Human Hepatic triacylglycerol lipase ELISA Kit; LIPC/HTGL/Hepatic triacylglycerol lipase/HL/Hepatic lipase/Lipase member C; LIPC elisa kit

• Reactivity: Human
• Sequence Length: 510

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LIPC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LIPC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LIPC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LIPC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LIPC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31982278
• NCBI GeneID: 15450
• NCBI Accession #: NP_032306.2
• NCBI GenBank Nucleotide #: NM_008280.2
• Molecular Weight: 57,389 Da
• NCBI Official Full Name: hepatic triacylglycerol lipase
• NCBI Official Synonym Full Names: lipase, hepatic
• NCBI Official Symbol: Lipc
• NCBI Official Synonym Symbols: HL; Hpl; AI256194
• NCBI Protein Information: hepatic triacylglycerol lipase
• UniProt Protein Name: Hepatic triacylglycerol lipase
• Protein Family: Hepatic triacylglycerol lipase
• UniProt Gene Name: Lipc
• UniProt Synonym Gene Names: Hpl; HL; Hepatic lipase
• UniProt Entry Name: LIPC_MOUSE

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; EC 3.1.1.3; Secreted; Lipid Metabolism - glycerolipid; Phospholipase

Cellular Component: cell surface; early endosome; extracellular region; extracellular space; late endosome; microvillus

Molecular Function: acylglycerol lipase activity; apolipoprotein binding; heparan sulfate proteoglycan binding; heparin binding; hydrolase activity; lipase activity; lipid binding; low-density lipoprotein binding; lysophospholipase activity; phospholipase A1 activity; protein binding; transferase activity, transferring acyl groups; triacylglycerol lipase activity

Biological Process: cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fatty acid biosynthetic process; fatty acid metabolic process; glycerophospholipid catabolic process; heparan sulfate proteoglycan biosynthetic process; lipid catabolic process; lipid metabolic process; neutral lipid catabolic process; phosphatidic acid metabolic process; phosphatidylcholine metabolic process; phosphatidylethanolamine metabolic process; phosphatidylserine metabolic process; protein oligomerization; triacylglycerol catabolic process; triacylglycerol metabolic process