Human Endothelial Lipase ELISA Kit | EL/LIPG elisa kit

Human Endothelial Lipase ELISA Kit

Cat. Num. AAA760082

• Gene Names: LIPG; EL; EDL; PRO719
• Reactivity: Human
• Synonyms: Endothelial Lipase; Human Endothelial Lipase ELISA Kit; EL/LIPG; EL/LIPG elisa kit

• Reactivity: Human
• Sequence Length: 500

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EL/LIPG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4836419
• NCBI GeneID: 9388
• Molecular Weight: 39,362 Da
• NCBI Official Full Name: endothelial lipase
• NCBI Official Synonym Full Names: lipase G, endothelial type
• NCBI Official Symbol: LIPG
• NCBI Official Synonym Symbols: EL; EDL; PRO719
• NCBI Protein Information: endothelial lipase
• UniProt Protein Name: Endothelial lipase
• Protein Family: Endothelial lipase
• UniProt Gene Name: LIPG
• UniProt Synonym Gene Names: EDL; EL
• UniProt Entry Name: LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Lipid Metabolism - glycerolipid; Phospholipase; Secreted, signal peptide; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cell surface; early endosome; extracellular region; extracellular space; Golgi apparatus

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; lipid digestion; lipid metabolic process; phospholipid catabolic process; phospholipid homeostasis; positive regulation of cholesterol transport; regulation of lipoprotein metabolic process; response to nutrient; reverse cholesterol transport