Rat Pancreatic triacylglycerol lipase ELISA Kit | Pnlip elisa kit

Rat Pancreatic triacylglycerol lipase ELISA Kit

Cat. Num. AAA763183

• Gene Names: PNLIP; PL; PTL; PNLIPD
• Reactivity: Rat
• Synonyms: Pancreatic triacylglycerol lipase; Rat Pancreatic triacylglycerol lipase ELISA Kit; Pnlip/Pancreatic triacylglycerol lipase/PL/PTL/Pancreatic lipase/PNLIPD; Pnlip elisa kit

• Reactivity: Rat
• Sequence Length: 465

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Pnlip elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Pnlip antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Pnlip antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Pnlip amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Pnlip can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10835000
• NCBI GeneID: 5406
• NCBI Accession #: NP_000927.1
• NCBI GenBank Nucleotide #: NM_000936.2
• Molecular Weight: 51,157 Da
• NCBI Official Full Name: pancreatic triacylglycerol lipase
• NCBI Official Synonym Full Names: pancreatic lipase
• NCBI Official Symbol: PNLIP
• NCBI Official Synonym Symbols: PL; PTL; PNLIPD
• NCBI Protein Information: pancreatic triacylglycerol lipase
• UniProt Protein Name: Pancreatic triacylglycerol lipase
• Protein Family: Pancreatic triacylglycerol lipase
• UniProt Gene Name: PNLIP
• UniProt Synonym Gene Names: PL; PTL; Pancreatic lipase
• UniProt Entry Name: LIPP_HUMAN

NCBI Description

This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Hydrolase; Lipid Metabolism - glycerolipid; EC 3.1.1.3; Secreted

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: extracellular region

Molecular Function: metal ion binding; triacylglycerol lipase activity

Biological Process: cholesterol absorption; fat-soluble vitamin metabolic process; lipid catabolic process; lipid digestion; phototransduction, visible light; retinoid metabolic process; vitamin metabolic process

Disease: Pancreatic Lipase Deficiency