Rat Matrix Metalloproteinase 3 ELISA Kit | MMP-3 elisa kit

Rat Matrix Metalloproteinase 3 ELISA Kit

Cat. Num. AAA762109

• Gene Names: MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• Reactivity: Rat
• Synonyms: Matrix Metalloproteinase 3; Rat Matrix Metalloproteinase 3 ELISA Kit; MMP-3 (Matrix Metalloproteinase 3)/Stromelysin 1/CHDS6/proteoglycanase/SL-1/STMY/STR1/stromelysin-1/transin-1/Transin-1; MMP-3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP-3 . No significant cross-reactivity or interference between MMP-3 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between MMP-3 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 477

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP-3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP-3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP-3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MMP-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MMP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 188619
• NCBI GeneID: 4314
• Molecular Weight: 53,977 Da
• NCBI Official Full Name: matrix metalloproteinase-3
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: MMP3
• NCBI Official Synonym Symbols: SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• NCBI Protein Information: stromelysin-1
• UniProt Protein Name: Stromelysin-1
• UniProt Gene Name: MMP3
• UniProt Synonym Gene Names: STMY1; SL-1; MMP-3
• UniProt Entry Name: MMP3_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.17; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular region; extracellular space

Molecular Function: endopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; positive regulation of protein oligomerization; proteolysis

Disease: Coronary Heart Disease, Susceptibility To, 6