Typical Testing Data/Standard Curve (for reference only)
Rat Fibronectin Leucine Rich Transmembrane Protein 2 ELISA Kit | FLRT2 elisa kit
Rat Fibronectin Leucine Rich Transmembrane Protein 2 ELISA Kit
Reactivity
Rat
Synonyms
Fibronectin Leucine Rich Transmembrane Protein 2; Rat Fibronectin Leucine Rich Transmembrane Protein 2 ELISA Kit; FLRT2 elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FLRT2 elisa kit
Intended Uses: This FTP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FTP2.
Principle of the Assay: FTP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FTP2 antibody and an FTP2-HRP conjugate. The assay sample and buffer are incubated together with FTP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTP2 concentration since FTP2 from samples and FTP2-HRP conjugate compete for the anti-FTP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FTP2 from the sample, fewer sites are left to bind FTP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTP2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FTP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FTP2 antibody and an FTP2-HRP conjugate. The assay sample and buffer are incubated together with FTP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTP2 concentration since FTP2 from samples and FTP2-HRP conjugate compete for the anti-FTP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FTP2 from the sample, fewer sites are left to bind FTP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FLRT2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
74,049 Da
NCBI Official Full Name
FLRT2
UniProt Protein Name
Leucine-rich repeat transmembrane protein FLRT2
Protein Family
UniProt Gene Name
FLRT2
UniProt Synonym Gene Names
KIAA0405; UNQ232/PRO265
UniProt Entry Name
FLRT2_HUMAN
Uniprot Description
FLRT2: May have a function in cell adhesion and/or receptor signaling.
Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q24-q32
Cellular Component: extracellular space; proteinaceous extracellular matrix; focal adhesion; integral to plasma membrane
Molecular Function: protein binding, bridging; chemorepellent activity; receptor signaling protein activity
Biological Process: axon guidance; negative chemotaxis; cell adhesion
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Product Notes
The Rat FLRT2 flrt2 (Catalog #AAA731126) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731126 ELISA Kit recognizes Rat FLRT2. It is sometimes possible for the material contained within the vial of "Fibronectin Leucine Rich Transmembrane Protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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