Rabbit MAP2K7 Polyclonal Antibody | anti-MAP2K7 antibody

MAP2K7 (Mitogen-activated Protein Kinase Kinase 7, Jnkk2, MAPKK7, MKK7, PRKMK7, SAPKK-4, SAPKK4,) (PE)

Cat. Num. AAA6425549

• Gene Names: MAP2K7; MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by affinity chromatography.
• Synonyms: MAP2K7; Polyclonal Antibody; MAP2K7 (Mitogen-activated Protein Kinase Kinase 7; Jnkk2; MAPKK7; MKK7; PRKMK7; SAPKK-4; SAPKK4; ) (PE); anti-MAP2K7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAP2K7. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAP2K7 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: ICC: 1:50-1:200; WB: 1:500-1:2000; IF: 1:50-1:200; IHC: 1:50-1:200; IP: 1:20-1:100; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MAP2K7.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MAP2K7 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using MAP2K7 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MAP2K7 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat spleen using MAP2K7 antibody at dilution of 1:100 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using MAP2K7 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-MAP2K7 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21735544
• NCBI GeneID: 5609
• NCBI Accession #: NP_660186.1
• NCBI GenBank Nucleotide #: NM_145185.2
• UniProt Accession #: O14733; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10; B2R9S5; D6W659
• Molecular Weight: 47,485 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7; MEK 7; JNKK 2; MAPKK 7; SAPK kinase 4; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; stress-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2
• UniProt Entry Name: MP2K7_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.

Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cytoplasm; intracellular; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein C-terminus binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; protein kinase binding; protein phosphatase binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; response to osmotic stress; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to heat; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; response to UV