Human Cyclin-dependent kinase 12 ELISA Kit | CDK12 elisa kit

Human Cyclin-dependent kinase 12 ELISA Kit

Cat. Num. AAA761602

• Gene Names: cdk12; crk7; crkr; crkrs
• Reactivity: Human
• Synonyms: Cyclin-dependent kinase 12; Human Cyclin-dependent kinase 12 ELISA Kit; CDK12/Cell division protein kinase 12 (hCDK12)/Cdc2-related kinase; arginine/serine-rich (CrkRS)/Cell division cycle 2-related protein kinase 7 (CDC2-related protein kinase 7)/; CDK12 elisa kit

• Reactivity: Human
• Sequence Length: 1239

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CDK12 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CDK12 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CDK12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CDK12 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman CDK12 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 198282055
• NCBI GeneID: 100038049
• NCBI Accession #: NP_001128283.1
• NCBI GenBank Nucleotide #: NM_001134811.1
• Molecular Weight: 138,328 Da
• NCBI Official Full Name: cyclin-dependent kinase 12
• NCBI Official Synonym Full Names: cyclin-dependent kinase 12
• NCBI Official Symbol: cdk12
• NCBI Official Synonym Symbols: crk7; crkr; crkrs
• NCBI Protein Information: cyclin-dependent kinase 12
• UniProt Protein Name: Cyclin-dependent kinase 12
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: cdk12
• UniProt Entry Name: CDK12_XENTR

Uniprot Description

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating srsf1/sf2 ().