Rabbit anti-Human TNFRSF6B Polyclonal Antibody | anti-TNFRSF6B antibody

TNFRSF6B, NT (TNFRSF6B, DCR3, TR6, Tumor necrosis factor receptor superfamily member 6B, Decoy receptor 3, Decoy receptor for Fas ligand, M68) (APC)

Cat. Num. AAA6357000

• Gene Names: TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TNFRSF6B; Polyclonal Antibody; NT (TNFRSF6B; DCR3; TR6; Tumor necrosis factor receptor superfamily member 6B; Decoy receptor 3; Decoy receptor for Fas ligand; M68) (APC); M68; anti-TNFRSF6B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNFRSF6B.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TNFRSF6B antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-48 in the N-terminal region of human TNFRSF6B, UniProt Accession #O95407; NP_116563.1; NP_003814.1
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of 293 cell line lysates (35ug/lane) usingMBS642741. This demonstrates that MBS642741 detected the TNFRSF6B protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human colon carcinoma using MBS642741 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells (right histogram) using MBS642741 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TNFRSF6B antibody

This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL-and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Overexpression of this gene has been noted in gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes. Two transcript variants encoding the same isoform, but differing in the 5' UTR, have been observed for this gene.

Product Categories/Family for anti-TNFRSF6B antibody

Antibodies; Receptors

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Xiong, G., et al. Tumour Biol. 31(5):443-449(2010). 3. Sung, H.Y., et al. Int. J. Radiat. Biol. 86(9):780-790(2010). 4. Brunetti, G., et al. Ann. N. Y. Acad. Sci. 1192, 298-302 (2010). 5. Perdigones, N., et al. Arthritis Rheum. 62(3):705-710(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 14790170
• NCBI GeneID: 8771
• NCBI Accession #: NP_116563.1; NP_003814.1
• NCBI GenBank Nucleotide #: NM_032945
• UniProt Accession #: O95407
• Molecular Weight: 32,680 Da
• NCBI Official Synonym Full Names: TNF receptor superfamily member 6b
• NCBI Official Symbol: TNFRSF6B
• NCBI Official Synonym Symbols: M68; TR6; DCR3; M68E; DJ583P15.1.1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6B
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF6B
• UniProt Synonym Gene Names: DCR3; TR6; DcR3
• UniProt Entry Name: TNF6B_HUMAN

NCBI Description

This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene, which encodes regulator of telomere elongation helicase 1 (RTEL1), generates a non-coding transcript. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFRSF6B: Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: extracellular space

Molecular Function: protein binding; receptor activity

Biological Process: apoptosis; negative regulation of apoptosis