Typical Testing Data/Standard Curve (for reference only)
Mouse Spink1 (Serine protease inhibitor Kazal-type 1) ELISA Kit | Spink1 elisa kit
Mouse Spink1 (Serine protease inhibitor Kazal-type 1) ELISA Kit
Gene Names
Spink1; p12; Spink3
Reactivity
Mouse
Synonyms
Spink1 (Serine protease inhibitor Kazal-type 1); Mouse Spink1 (Serine protease inhibitor Kazal-type 1) ELISA Kit; Serine protease inhibitor Kazal-type 1; P12; Prostatic secretory glycoprotein; Serine protease inhibitor Kazal-type 3; Spink1; Spink3; Spink1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Spink1. No significant cross-reactivity or interference between Spink1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 2-8 degree C for 6 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Spink1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33.1 kDa
NCBI Official Full Name
serine protease inhibitor Kazal-type 1
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 1
NCBI Official Symbol
Spink1
NCBI Official Synonym Symbols
p12; Spink3
NCBI Protein Information
serine protease inhibitor Kazal-type 1
UniProt Protein Name
Serine protease inhibitor Kazal-type 1
Protein Family
UniProt Gene Name
Spink1
UniProt Entry Name
ISK1_MOUSE
Uniprot Description
Spink3: Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa. By androgens in adult male sex accessory glands but expressed constitutively in pancreas
Protein type: Inhibitor
Cellular Component: acrosome; extracellular region; extracellular space
Molecular Function: protease inhibitor activity; serine-type endopeptidase inhibitor activity
Biological Process: negative regulation of peptidase activity; negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction
Research Articles on Spink1
Similar Products
Product Notes
The Mouse Spink1 spink1 (Catalog #AAA7612401) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7612401 ELISA Kit recognizes Mouse Spink1. It is sometimes possible for the material contained within the vial of "Spink1 (Serine protease inhibitor Kazal-type 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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