Human Pancreatic secretory trypsin inhibitor ELISA Kit | SPINK1/TATI elisa kit

Human Pancreatic secretory trypsin inhibitor ELISA Kit

Cat. Num. AAA763970

• Gene Names: SPINK1; TCP; PCTT; PSTI; TATI; Spink3
• Reactivity: Human
• Synonyms: Pancreatic secretory trypsin inhibitor; Human Pancreatic secretory trypsin inhibitor ELISA Kit; SPINK1 (pancreatic secretory trypsin inhibitor)/TATI/PCTT/PSTI/Spink3; SPINK1/TATI elisa kit

• Reactivity: Human
• Sequence Length: 79

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SPINK1/TATI elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SPINK1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SPINK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPINK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SPINK1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 190688
• NCBI GeneID: 6690
• Molecular Weight: 8,507 Da
• NCBI Official Full Name: pancreatic secretory trypsin inhibitor
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 1
• NCBI Official Symbol: SPINK1
• NCBI Official Synonym Symbols: TCP; PCTT; PSTI; TATI; Spink3
• NCBI Protein Information: serine protease inhibitor Kazal-type 1
• UniProt Protein Name: Serine protease inhibitor Kazal-type 1
• Protein Family: Pancreatic secretory trypsin inhibitor
• UniProt Gene Name: SPINK1
• UniProt Synonym Gene Names: PSTI; TATI
• UniProt Entry Name: ISK1_HUMAN

NCBI Description

The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]

Uniprot Description

SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: acrosome; extracellular space

Molecular Function: endopeptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction

Disease: Pancreatitis, Hereditary; Tropical Calcific Pancreatitis