Mouse Phospholipase A2, Group IID ELISA Kit | PLA2G2D elisa kit

Mouse Phospholipase A2, Group IID ELISA Kit

Cat. Num. AAA761911

• Gene Names: PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
• Reactivity: Mouse
• Synonyms: Phospholipase A2; Group IID; Mouse Phospholipase A2; Group IID ELISA Kit; PLA2G2D; PLA2G2D elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLA2G2D. No significant cross-reactivity or interference between PLA2G2D and analogues was observed.
• Sequence Length: 145

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G2D elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119615321
• NCBI GeneID: 26279
• Molecular Weight: 16,546 Da
• NCBI Official Full Name: phospholipase A2, group IID
• NCBI Official Synonym Full Names: phospholipase A2 group IID
• NCBI Official Symbol: PLA2G2D
• NCBI Official Synonym Symbols: SPLASH; sPLA2S; PLA2IID; sPLA2-IID
• NCBI Protein Information: group IID secretory phospholipase A2
• UniProt Protein Name: Group IID secretory phospholipase A2
• Protein Family: Group IID secretory phospholipase
• UniProt Gene Name: PLA2G2D
• UniProt Synonym Gene Names: SPLASH; GIID sPLA2; sPLA2-IID
• UniProt Entry Name: PA2GD_HUMAN

NCBI Description

This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Secreted; Phospholipase; Secreted, signal peptide; EC 3.1.1.4

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular region

Molecular Function: calcium ion binding; heparan sulfate proteoglycan binding; heparin binding; phospholipase A2 activity

Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; glycerophospholipid biosynthetic process; inflammatory response; lipid catabolic process; negative regulation of T cell proliferation; phosphatidic acid biosynthetic process; phospholipid metabolic process