Rat AKR1B1 (Aldose reductase) ELISA Kit | AKR1B1 elisa kit

Rat AKR1B1 (Aldose reductase) ELISA Kit

Cat. Num. AAA7606282

• Gene Names: Akr1b1; Alr; Aldr1; ALDRED; Akr1b3; Akr1b4; ALR-P-I; RATALDRED
• Reactivity: Rat
• Synonyms: AKR1B1 (Aldose reductase); Rat AKR1B1 (Aldose reductase) ELISA Kit; AKR1B1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of AKR1B1. No significant cross-reactivity or interference between AKR1B1 and analogues was observed.
• Sequence Length: 316

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AKR1B1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AKR1B1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AKR1B1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AKR1B1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AKR1B1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AKR1B1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AKR1B1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1168407
• NCBI GeneID: 24192
• UniProt Accession #: P07943
• Molecular Weight: 35,797 Da
• NCBI Official Full Name: Aldose reductase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member B
• NCBI Official Symbol: Akr1b1
• NCBI Official Synonym Symbols: Alr; Aldr1; ALDRED; Akr1b3; Akr1b4; ALR-P-I; RATALDRED
• NCBI Protein Information: aldose reductase
• UniProt Protein Name: Aldose reductase
• Protein Family: Aldose reductase
• UniProt Gene Name: Akr1b1
• UniProt Synonym Gene Names: Akr1b4; Aldr1; AR

NCBI Description

regulates NF-kappa B mediated mitogenic signaling; may play a role in the polyol pathway [RGD, Feb 2006]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: cytosol; extracellular space; mast cell granule; nucleoplasm; perinuclear region of cytoplasm

Molecular Function: aldehyde reductase activity; glyceraldehyde oxidoreductase activity

Biological Process: maternal process involved in pregnancy; monosaccharide metabolic process; naphthalene metabolic process; negative regulation of apoptosis; norepinephrine metabolic process; positive regulation of JAK-STAT cascade; positive regulation of smooth muscle cell proliferation; renal water homeostasis; response to organic substance; response to water deprivation; sorbitol biosynthetic process; stress-activated protein kinase signaling pathway; tissue homeostasis