Rabbit anti-Human IKZF1 Polyclonal Antibody | anti-IKZF1 antibody

IKZF1, CT (IKZF1, IK1, IKAROS, LYF1, ZNFN1A1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1) (MaxLight 750)

Cat. Num. AAA6309825

• Gene Names: IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IKZF1; Polyclonal Antibody; CT (IKZF1; IK1; IKAROS; LYF1; ZNFN1A1; DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1) (MaxLight 750); Lymphoid transcription factor LyF-1; anti-IKZF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IKZF1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-IKZF1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 415-444 in the C-terminal region of human IKZF1, UniProt Accession #NP_006051.1; Q13422.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6004818. This demonstrates that MBS6004818 detected the IKZF1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of extracts from MM cells, treated with DMSO or lenalidomide, usingMBS6004818.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS6004818 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004818 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of CEM cells using MBS6004818 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IKZF1 antibody

MaxLight750 is a new Near IR stable dye conjugate comparable to DyLight750, Alexa Fluor700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.

Product Categories/Family for anti-IKZF1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. He, C.F., et al. Lupus 19(10):1181-1186(2010). 2. Yang, L., et al. Oncol. Rep. 24(2):571-577(2010). 3. Jager, R., et al. Leukemia 24(7):1290-1298(2010). 4. Kuiper, R.P., et al. Leukemia 24(7):1258-1264(2010). 5. Matulic, M., et al. Coll Antropol 34(1):59-62(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5174501
• NCBI GeneID: 10320
• NCBI Accession #: NP_006051.1
• NCBI GenBank Nucleotide #: NM_006060.5
• UniProt Accession #: Q13422; O00598; Q53XL2; Q69BM4; Q8WVA3; A4D260; B4E0Z1; D3DVM5
• Molecular Weight: 24,470 Da
• NCBI Official Full Name: DNA-binding protein Ikaros isoform 1
• NCBI Official Synonym Full Names: IKAROS family zinc finger 1 (Ikaros)
• NCBI Official Symbol: IKZF1
• NCBI Official Synonym Symbols: IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452
• NCBI Protein Information: DNA-binding protein Ikaros; CLL-associated antigen KW-6; ikaros family zinc finger protein 1; lymphoid transcription factor LyF-1; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros)
• UniProt Protein Name: DNA-binding protein Ikaros
• Protein Family: DNA-binding protein
• UniProt Gene Name: IKZF1
• UniProt Synonym Gene Names: IK1; IKAROS; LYF1; ZNFN1A1
• UniProt Entry Name: IKZF1_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Uniprot Description

Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity

Biological Process: Peyer's patch development; thymus development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of multicellular organism growth; chromatin modification; cell cycle; lymph node development; positive regulation of neutrophil differentiation; retina development in camera-type eye; natural killer cell differentiation; B cell differentiation; forebrain development; positive regulation of NK T cell differentiation; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation