Rat Aldose reductase ELISA Kit | Akr1b1 elisa kit

Rat Aldose reductase ELISA Kit

Cat. Num. AAA2881662

• Gene Names: Akr1b1; Alr; Aldr1; ALDRED; Akr1b3; Akr1b4; ALR-P-I; RATALDRED
• Reactivity: Rat
• Synonyms: Aldose reductase; Rat Aldose reductase ELISA Kit; AR; Aldehyde reductase; Akr1b1; Akr1b4; Aldr1; 1.1.1.21; Akr1b1 elisa kit

• Reactivity: Rat
• Sequence Length: 316

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Akr1b1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978491
• NCBI GeneID: 24192
• NCBI Accession #: NP_036630.1
• NCBI GenBank Nucleotide #: NM_012498.1
• UniProt Accession #: P07943
• Molecular Weight: 35,797 Da
• NCBI Official Full Name: aldose reductase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member B1 (aldose reductase)
• NCBI Official Symbol: Akr1b1
• NCBI Official Synonym Symbols: Alr; Aldr1; ALDRED; Akr1b3; Akr1b4; ALR-P-I; RATALDRED
• NCBI Protein Information: aldose reductase
• UniProt Protein Name: Aldose reductase
• Protein Family: Aldose reductase
• UniProt Gene Name: Akr1b1
• UniProt Synonym Gene Names: Akr1b4; Aldr1; AR
• UniProt Entry Name: ALDR_RAT

NCBI Description

regulates NF-kappa B mediated mitogenic signaling; may play a role in the polyol pathway [RGD, Feb 2006]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - glycerolipid; Oxidoreductase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.21; Carbohydrate Metabolism - fructose and mannose

Cellular Component: cytoplasm; cytosol; extracellular space; mast cell granule; nucleoplasm; perinuclear region of cytoplasm

Molecular Function: aldehyde reductase activity; glyceraldehyde oxidoreductase activity

Biological Process: maternal process involved in pregnancy; monosaccharide metabolic process; naphthalene metabolic process; norepinephrine metabolic process; positive regulation of JAK-STAT cascade; positive regulation of smooth muscle cell proliferation; response to organic substance; response to water deprivation; sorbitol biosynthetic process; stress-activated protein kinase signaling pathway; tissue homeostasis