Mouse NLRP3 (Nod Like Receptor Pyrins-3) ELISA Kit | NLRP3 elisa kit

Mouse NLRP3 (Nod Like Receptor Pyrins-3) ELISA Kit

Cat. Num. AAA7606270

• Gene Names: Nlrp3; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• Reactivity: Mouse
• Synonyms: NLRP3 (Nod Like Receptor Pyrins-3); Mouse NLRP3 (Nod Like Receptor Pyrins-3) ELISA Kit; NLRP3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of NLRP3. No significant cross-reactivity or interference between NLRP3 and analogues was observed.
• Sequence Length: 1033

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for NLRP3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 29427844
• NCBI GeneID: 216799
• UniProt Accession #: Q8R4B8; Q1JQ87; Q1JQ88; Q6JEL0; T1W2H6
• Molecular Weight: 95,710 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: Nlrp3
• NCBI Official Synonym Symbols: FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: Nlrp3
• UniProt Synonym Gene Names: Cias1; Mmig1; Nalp3; Pypaf1

Uniprot Description

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11|11 B1.3

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; sequence-specific DNA binding; transcription factor binding

Biological Process: activation of NF-kappaB transcription factor; caspase activation; defense response to virus; inflammatory response; inhibition of NF-kappaB transcription factor; interleukin-1 beta production; interleukin-1 secretion; interleukin-18 production; negative regulation of acute inflammatory response; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-5 production; positive regulation of T-helper 2 cell differentiation; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter; regulation of inflammatory response