Rabbit anti-Mouse Nlrp3 Polyclonal Antibody | anti-Nlrp3 antibody

Nlrp3 Antibody

Cat. Num. AAA7044169

• Gene Names: Nlrp3; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• Reactivity: Mouse
• Applications: ELISA, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: Nlrp3; Polyclonal Antibody; Nlrp3 Antibody; NACHT; LRR and PYD domains-containing protein 3; Cold autoinflammatory syndrome 1 protein homolog; Cryopyrin; Mast cell maturation-associated-inducible protein 1; PYRI; Cias1; Mmig1; Nalp3; Pypaf1; anti-Nlrp3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1033

• Applicable Applications for anti-Nlrp3 antibody: ELISA (EIA), Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:200
• Immunogen: Recombinant mouse NACHT, LRR and PYD domains-containing protein 3 protein (1-153AA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Species: Mus musculus (Mouse)
• Research Area: Immunology
• Target Names: Nlrp3
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7044169 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-Nlrp3 antibody

Nlrp3 polyclonal antibody MBS7044169 was produced in the rabbit immunized by using the Recombinant Mouse NACHT, LRR and PYD domains- containing protein 3 protein (1-153AA) as the immunogen. The target protein Nlrp3 plays a crucial role in innate immunity and inflammation as the sensor component of the NLRP3 inflammasome. In response to pathogens and other damageassociated signals, it can initiate the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). This Rabbit anti-Mus musculus (Mouse) Nlrp3 Polyclonal antibody was tested in the ELISA and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the Nlrp3 proteins of humanorigin and may be used to detect the endogenous levels of Nlrp3 protein.

NCBI and Uniprot Product Information

• NCBI GI #: 22003870
• NCBI GeneID: 216799
• NCBI Accession #: NP_665826.1
• NCBI GenBank Nucleotide #: NM_145827.3
• UniProt Accession #: Q8R4B8; Q1JQ87; Q1JQ88; Q6JEL0; T1W2H6
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: Nlrp3
• NCBI Official Synonym Symbols: FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: Nlrp3
• UniProt Synonym Gene Names: Cias1; Mmig1; Nalp3; Pypaf1
• UniProt Entry Name: NLRP3_MOUSE

Uniprot Description

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor binding

Biological Process: activation of NF-kappaB transcription factor; caspase activation; defense response to virus; inflammatory response; inhibition of NF-kappaB transcription factor; interleukin-1 beta production; interleukin-1 secretion; interleukin-18 production; negative regulation of acute inflammatory response; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-5 production; positive regulation of T-helper 2 cell differentiation; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter; regulation of inflammatory response