Mouse NACHT, LRR and PYD Domains-Containing Protein 3 (NLRP3) RTU ELISA Kit | NLRP3 rtu elisa kit

Mouse NACHT, LRR and PYD Domains-Containing Protein 3 (NLRP3) ELISA Kit

Cat. Num. AAA4503892

• Gene Names: Nlrp3; FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• Reactivity: Mouse
• Synonyms: NACHT; LRR and PYD Domains-Containing Protein 3 (NLRP3); Mouse NACHT; LRR and PYD Domains-Containing Protein 3 (NLRP3) ELISA Kit; PYRIN-containing APAF1-like protein 1; Cryopyrin; Cold autoinflammatory syndrome 1 protein; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1(CLR1.1); NLRP3 rtu elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of NLRP3. No significant cross-reactivity or interference between NLRP3 and analogues was observed.
• Sequence Length: 1033

• Samples: Mouse Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLRP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLRP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NLRP3 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NLRP3 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLRP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NLRP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 29427844
• NCBI GeneID: 216799
• UniProt Accession #: Q8R4B8
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: Nlrp3
• NCBI Official Synonym Symbols: FCU; MWS; FCAS; Cias1; Mmig1; NALP3; Pypaf1; AII/AVP; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: Nlrp3
• UniProt Synonym Gene Names: Cias1; Mmig1; Nalp3; Pypaf1
• UniProt Entry Name: NALP3_MOUSE

Uniprot Description

Function: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA

By similarity. Activates caspase-1 as part of the NALP3 inflammasome complex in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure: Interacts with PYCARD/ASC. Part of the NALP3 inflammasome complex which is involved in activation of caspase-1 and caspase-5, leading to processing of IL1B and IL18

By similarity. Interacts with PML

By similarity. Interacts with EIF2AK2/PKR. Ref.10

Subcellular location: Cytoplasm Ref.1.

Tissue specificity: Widely expressed with high levels in peripheral blood leukocytes. Not detected in thymus. Ref.2 Ref.6

Disruption phenotype: Mice display lack of activation of caspase-1 in response to stimuli such as LPS and ATP, viral infection, bacterial and viral RNA, non-viral double-stranded RNA, nigericin, maitotoxin and the imidazoquinoline compounds R837 and R848. Ref.6 Ref.7 Ref.8 Ref.9

Miscellaneous: Expression is increased in mice with experimental atopic dermatitis.

Sequence similarities: Belongs to the NLRP family.Contains 1 DAPIN domain.Contains 9 LRR (leucine-rich) repeats.Contains 1 NACHT domain.