Mouse SIRT2 (Sirtuin 2) ELISA Kit | SIRT2 elisa kit

Mouse SIRT2 (Sirtuin 2) ELISA Kit

Cat. Num. AAA7606185

• Gene Names: Sirt2; Sir2l; SIR2L2; 5730427M03Rik
• Reactivity: Mouse
• Synonyms: SIRT2 (Sirtuin 2); Mouse SIRT2 (Sirtuin 2) ELISA Kit; Sirt2; SIR2L2; sirtuin 2; sirtuin type 2; NAD-dependent deacetylase sirtuin-2; SIR2-like protein 2; SIRT2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SIRT2. No significant cross-reactivity or interference between SIRT2 and analogues was observed.
• Sequence Length: 389

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIRT2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38258618
• NCBI GeneID: 64383
• UniProt Accession #: Q8VDQ8; Q9CXS5; Q9EQ18; Q9ERJ9; E9PXF5; U5TP50
• Molecular Weight: 35,678 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-2
• NCBI Official Synonym Full Names: sirtuin 2
• NCBI Official Symbol: Sirt2
• NCBI Official Synonym Symbols: Sir2l; SIR2L2; 5730427M03Rik
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-2
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-2
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: Sirt2
• UniProt Synonym Gene Names: Sir2l2; mSIR2L2

Uniprot Description

SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 7|7 B1

Cellular Component: centriole; centrosome; chromosome; cytoplasm; cytosol; lateral loop; microtubule; midbody; mitochondrion; myelin sheath; nuclear heterochromatin; nucleolus; nucleus; paranode region of axon; perikaryon; perinuclear region of cytoplasm; plasma membrane; spindle

Molecular Function: beta-tubulin binding; chromatin binding; histone acetyltransferase binding; histone deacetylase activity; histone deacetylase binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); protein binding; protein deacetylase activity; transcription factor binding; tubulin deacetylase activity; ubiquitin binding; zinc ion binding

Biological Process: cellular lipid catabolic process; hepatocyte growth factor receptor signaling pathway; histone deacetylation; myelination in the peripheral nervous system; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of protein catabolic process; negative regulation of striated muscle development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; phosphoinositide 3-kinase cascade; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of cell division; positive regulation of DNA binding; positive regulation of meiosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid deacetylation; protein kinase B signaling cascade; regulation of cell cycle; regulation of fat cell differentiation; regulation of myelination