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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue slide using MBS7603545 (RIP3 antibody) at dilution of 1:200)

Rabbit anti-Human, Mouse RIP3 Polyclonal Antibody | anti-RIP3 antibody

anti-RIP3 antibody

Reactivity
Human, Mouse
Applications
ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Synonyms
RIP3; Polyclonal Antibody; anti-RIP3 antibody; Receptor interacting protein 3; RIP 3; RIP like protein kinase 3; RIPK3
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Applicable Applications for anti-RIP3 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC).
Application Notes
WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:100-1:400
Immunogen
receptor-interacting serine-threonine kinase 3
Preparation and Storage
-20 degree C for 12 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas tissue slide using MBS7603545 (RIP3 antibody) at dilution of 1:200)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue slide using MBS7603545 (RIP3 antibody) at dilution of 1:200)

Immunoprecipitation (IP)

(IP Result of anti-RIP3 (IP: MBS7603545, 3ug; Detection: MBS7603545 1:300) with SW 1990 cells lysate 3000ug.)

Immunoprecipitation (IP) (IP Result of anti-RIP3 (IP: MBS7603545, 3ug; Detection: MBS7603545 1:300) with SW 1990 cells lysate 3000ug.)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7603545 (RIP3 antibody) at dilution of 1:500)

Western Blot (WB) (Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7603545 (RIP3 antibody) at dilution of 1:500)
Related Product Information for anti-RIP3 antibody
Essential for necroptosis, a programmed cell death process in response to death-inducing TNFalpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
57 kDa

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Product Notes

The RIP3 (Catalog #AAA7603545) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The anti-RIP3 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RIP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC). . WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:100-1:400. Researchers should empirically determine the suitability of the RIP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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