Mouse anti-Human RIPK3 Monoclonal Antibody | anti-RIPK3 antibody

RIPK3 Antibody

Cat. Num. AAA9231337

• Gene Names: RIPK3; RIP3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified through a protein G column, followed by dialysis against PBS.
• Synonyms: RIPK3; Monoclonal Antibody; RIPK3 Antibody; Receptor-interacting serine/threonine-protein kinase 3; 2.7.11.1; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; anti-RIPK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 2013CT892.86.49
• Specificity: This RIPK3 antibody is generated from a mouse immunized with a recombinate protein from the human region of human RIPK3.
• Purity/Purification: Purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 518

• Applicable Applications for anti-RIPK3 antibody: Western Blot (WB), ELISA (EIA)
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Dilution: WB - 1:1000All lanes: Anti-RIPK3 Antibody at 1:1000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: SW620 whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RIPK3 antibody

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Product Categories/Family for anti-RIPK3 antibody

Signal Transduction

References

Yu P.W.,et al.Curr. Biol. 9:539-542(1999). Sun X.,et al.J. Biol. Chem. 274:16871-16875(1999). Yang Y.,et al.Biochem. Biophys. Res. Commun. 332:181-187(2005). Heilig R.,et al.Nature 421:601-607(2003). Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 205371831
• NCBI GeneID: 11035
• UniProt Accession #: Q9Y572
• Molecular Weight: 56887 Da
• NCBI Official Full Name: Receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor interacting serine/threonine kinase 3
• NCBI Official Symbol: RIPK3
• NCBI Official Synonym Symbols: RIP3
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK3
• UniProt Synonym Gene Names: RIP3; RIP-3
• UniProt Entry Name: RIPK3_HUMAN

NCBI Description

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; plasma membrane; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity