Bovine Annexin A1 ELISA Kit | ANXA1 elisa kit

Bovine Annexin A1 ELISA Kit

Cat. Num. AAA756020

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Bovine
• Synonyms: Annexin A1; Bovine Annexin A1 ELISA Kit; ANXA1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ANXA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ANXA1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for ANXA1 elisa kit

Princple of the assay: ANXA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA1 antibody and an ANXA1-HRP conjugate. The assay sample and buffer are incubated together with ANXA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA1 concentration since ANXA1 from samples and ANXA1-HRP conjugate compete for the anti-ANXA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA1 from the sample, fewer sites are left to bind ANXA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANXA1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.1
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN

NCBI Description

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: extracellular space; focal adhesion; mast cell granule; cell surface; protein complex; basolateral plasma membrane; extracellular region; cornified envelope; cilium; mitochondrial membrane; cytoplasm; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; protein binding; single-stranded RNA binding; protein homodimerization activity; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; helicase activity; calcium-dependent protein binding; single-stranded DNA binding; receptor binding

Biological Process: response to drug; prostate gland development; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; neutrophil homeostasis; signal transduction; arachidonic acid secretion; endocrine pancreas development; DNA duplex unwinding; response to estradiol stimulus; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; inflammatory response; cell motility; negative regulation of apoptosis; positive regulation of prostaglandin biosynthetic process; response to X-ray