Monkey Kruppel Like Factor 15 ELISA Kit | KLF15 elisa kit

Monkey Kruppel Like Factor 15 ELISA Kit

Cat. Num. AAA750431

• Gene Names: KLF15; KKLF
• Reactivity: Monkey
• Synonyms: Kruppel Like Factor 15; Monkey Kruppel Like Factor 15 ELISA Kit; KLF15 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for KLF15 elisa kit

Principle of the assay: KLF15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLF15 antibody and an KLF15-HRP conjugate. The assay sample and buffer are incubated together with KLF15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF15 concentration since KLF15 from samples and KLF15-HRP conjugate compete for the anti-KLF15 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF15 from the sample, fewer sites are left to bind KLF15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF15 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for KLF15 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 7662494
• NCBI GeneID: 28999
• NCBI Accession #: NP_054798.1
• NCBI GenBank Nucleotide #: NM_014079.3
• Molecular Weight: 43,992 Da
• NCBI Official Full Name: Krueppel-like factor 15
• NCBI Official Synonym Full Names: Kruppel-like factor 15
• NCBI Official Symbol: KLF15
• NCBI Official Synonym Symbols: KKLF
• NCBI Protein Information: Krueppel-like factor 15; kidney-enriched Kruppel-like factor; kidney-enriched krueppel-like factor
• UniProt Protein Name: Krueppel-like factor 15
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF15
• UniProt Synonym Gene Names: KKLF
• UniProt Entry Name: KLF15_HUMAN

Uniprot Description

KLF15: Transcriptional activator. Binds to the GA element of the CLCNKA promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: cardiac muscle hypertrophy; glial cell differentiation; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose transport