Typical Testing Data/Standard Curve (for reference only)
Sheep Anti-Actin Antibody ELISA Kit | AAA elisa kit
Sheep Anti-Actin Antibody ELISA Kit
Gene Names
Act5C; A; A4V404_DROME; Ac5C; act; Act; ACT; act 5C; Act-5C; ACT1_DROME; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; chrX:5748184..5748304; cyt5C; DmelCG4027; l(1)G00
Reactivity
Sheep
Synonyms
Anti-Actin Antibody; Sheep Anti-Actin Antibody ELISA Kit; AAA elisa kit
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Intended Uses
This anti-AAA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep anti-AAA. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This anti-AAA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep anti-AAA. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AAA elisa kit
Principle of the Assay: AAA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Actin antigen and an anti-AAA-HRP conjugate. The assay sample and buffer are incubated together with anti-AAA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-AAA concentration since anti-AAA from samples and anti-AAA-HRP conjugate compete for the Actin antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-AAA from the sample, fewer sites are left to bind anti-AAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-AAA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AAA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,822 Da
NCBI Official Full Name
actin 5C, isoform C
NCBI Official Synonym Full Names
Actin 5C
NCBI Official Symbol
Act5C
NCBI Official Synonym Symbols
A; A4V404_DROME; Ac5C; act; Act; ACT; act 5C; Act-5C; ACT1_DROME; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; chrX:5748184..5748304; cyt5C; DmelCG4027; l(1)G00
NCBI Protein Information
CG4027 gene product from transcript CG4027-RE; 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; Act5C-PE; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; CG4027-PE; Dm-actin5C; F-actin; G-actin; actin 5 C; actin 5C; actin 5c; actin A1; anon-fast-evolving-2D2; beta-actin; cellular cytoskeletal beta-actin
UniProt Protein Name
Actin-5C
UniProt Gene Name
Act5C
UniProt Synonym Gene Names
CG4027
UniProt Entry Name
ACT1_DROME
Uniprot Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Articles on AAA
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Product Notes
The Sheep AAA act5c (Catalog #AAA746828) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746828 ELISA Kit recognizes Sheep AAA. It is sometimes possible for the material contained within the vial of "Anti-Actin Antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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