Immunohistochemistry (IHC)
(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human Actin, pan Polyclonal Antibody | anti-Act5C antibody
Actin, pan, CT
Gene Names
Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Actin; pan; Polyclonal Antibody; CT; Anti -Actin; anti-Act5C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human Actin. Species sequence homology: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-Act5C antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Dilution: ELISA: 1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunogen
Synthetic peptide corresponding to human Actin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Actin-pan Antibody. The lane on the right is treated with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Actin-pan Antibody. The lane on the right is treated with the synthesized peptide.)
Product Categories/Family for anti-Act5C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,822 Da
NCBI Official Full Name
actin 5C, isoform C
NCBI Official Synonym Full Names
Actin 5C
NCBI Official Symbol
Act5C
NCBI Official Synonym Symbols
A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0
NCBI Protein Information
CG4027 gene product from transcript CG4027-RC; 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; Dm-actin5C; F-actin; G-actin; actin 5 C; actin 5C; actin 5c; actin A1; anon-fast-evolving-2D2; cellular cytoskeletal beta-actin
UniProt Protein Name
Actin-5C
Protein Family
UniProt Gene Name
Act5C
UniProt Entry Name
ACT1_DROME
Uniprot Description
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
Subcellular location: Cytoplasm › cytoskeleton.
Post-translational modification: Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
Miscellaneous: In Drosophila there are 6 closely related actin genes.
Sequence similarities: Belongs to the actin family.
Research Articles on Act5C
Similar Products
Product Notes
The Act5C act5c (Catalog #AAA6004961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin, pan, CT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, pan can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml. Researchers should empirically determine the suitability of the Act5C act5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, pan, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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