Chicken Anti-Muscle, Skeletal, receptor tyrosine kinase Antibody ELISA Kit | MUSK elisa kit

Chicken Anti-Muscle, Skeletal, receptor tyrosine kinase Antibody ELISA Kit

Cat. Num. AAA744889

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Reactivity: Chicken
• Synonyms: Anti-Muscle; Skeletal; receptor tyrosine kinase Antibody; Chicken Anti-Muscle; receptor tyrosine kinase Antibody ELISA Kit; MUSK elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUSK elisa kit

Intended Uses: This Anti-MUSK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken Anti-MUSK. This ELISA kit for research use only!

Principle of the Assay: Anti-MUSK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Muscle, Skeletal, receptor tyrosine kinase antigen. Standards or samples are then added to the microtiter plate wells and Anti-MUSK if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of Anti-MUSK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Muscle, Skeletal, receptor tyrosine kinase antigen is added to each well to "sandwich" the Anti-MUSK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-MUSK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-MUSK concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MUSK elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 394933752
• NCBI GeneID: 18198
• Molecular Weight: 98,081 Da
• NCBI Official Full Name: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase; muscle localized kinase 2; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine protein kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal receptor tyrosine protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Musk family

Cellular Component: postsynaptic membrane; cell projection; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction; neuromuscular junction; receptor complex; external side of plasma membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; ATP binding; PDZ domain binding; protein kinase activity

Biological Process: positive regulation of synaptic transmission, cholinergic; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; multicellular organismal development; positive regulation of synaptic growth at neuromuscular junction; signal transduction; protein amino acid phosphorylation; receptor clustering; memory; positive regulation of peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; cell differentiation; response to electrical stimulus; phosphorylation; neuromuscular junction development