Rabbit MOBP Polyclonal Antibody | anti-MOBP antibody

MOBP Polyclonal Antibody

Cat. Num. AAA3015206

• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: MOBP; Polyclonal Antibody; MOBP Polyclonal Antibody; MGC87379; Myelin associated oligodendrocyte basic protein; MOBP_HUMAN; anti-MOBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
• Clonality: Polyclonal
• Specificity: MOBP Polyclonal Antibody detects endogenous levels of MOBP protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-MOBP antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human MOBP
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Positive sample?Paraformaldehyde-fixed, paraffin embedded (mouse brian); Negative sample?Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Positive sample?Paraformaldehyde-fixed, paraffin embedded (rat brian); Negative sample?Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-MOBP antibody

The gene encoding MOBP a member of the central nervous system myelin-constituting proteins, maps to chromosome 3p22.2. MOBP has many splice variants that share a 68 amino acid N-terminal domain. MOBP-71, MOBP-81A, MOBP-99, and MOBP-169 are MOBP splice variants that contain exon 8b, which is similar to myelin basic protein (MBP) mRNA RTS, however MOBP-69, MOBP-81B, and MOBP-170 lack this exon. The splice variants that contain exon 8b are expressed in myelin, while those lacking exon 8b are retained in the oligodendrocyte soma. Exon 8b-containing variants are directed to sites of myelin sheath assembly by exon 8b, where they play a structural role in myelin formation. Splice variants lacking exon 8b likely play a cellular and/or regulatory role. MOBP is implicated in multiple sclerosis (MS), a human demyelinating disease, and in allergic encephalomyelitis in rodents.

NCBI and Uniprot Product Information

• NCBI GI #: 134034171
• NCBI GeneID: 4336
• UniProt Accession #: Q13875; Q13874; Q6DHZ6; Q8TBJ1; A8K2C2; G5E945
• Molecular Weight: 183
• NCBI Official Full Name: Myelin-associated oligodendrocyte basic protein
• NCBI Official Synonym Full Names: myelin-associated oligodendrocyte basic protein
• NCBI Official Symbol: MOBP
• NCBI Protein Information: myelin-associated oligodendrocyte basic protein
• UniProt Protein Name: Myelin-associated oligodendrocyte basic protein
• Protein Family: Myelin-associated oligodendrocyte basic protein
• UniProt Gene Name: MOBP
• UniProt Entry Name: MOBP_HUMAN

Uniprot Description

MOBP: May play a role in compacting or stabilizing the myelin sheath, possibly by binding the negatively charged acidic phospholipids of the cytoplasmic membrane. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm

Molecular Function: structural constituent of myelin sheath

Biological Process: nervous system development