Human Muscle, skeletal, receptor tyrosine kinase ELISA Kit | MuSK elisa kit

Human Muscle, skeletal, receptor tyrosine kinase ELISA Kit

Cat. Num. AAA725649

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Reactivity: Human
• Synonyms: Muscle; skeletal; receptor tyrosine kinase; Human Muscle; receptor tyrosine kinase ELISA Kit; MuSK elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for MuSK elisa kit

Intended Uses: This MUSK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MUSK. This ELISA kit for research use only!

Principle of the Assay: MUSK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MUSK antibody and an MUSK-HRP conjugate. The assay sample and buffer are incubated together with MUSK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUSK concentration since MUSK from samples and MUSK-HRP conjugate compete for the anti-MUSK antibody binding site. Since the number of sites is limited, as more sites are occupied by MUSK from the sample, fewer sites are left to bind MUSK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUSK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 394933752
• NCBI GeneID: 18198
• Molecular Weight: 98,081 Da
• NCBI Official Full Name: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase; muscle localized kinase 2; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine protein kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal receptor tyrosine protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Musk family

Cellular Component: postsynaptic membrane; cell projection; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction; neuromuscular junction; receptor complex; external side of plasma membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; protein kinase binding; ATP binding; PDZ domain binding; protein kinase activity

Biological Process: positive regulation of synaptic transmission, cholinergic; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of synaptic growth at neuromuscular junction; signal transduction; protein amino acid phosphorylation; receptor clustering; memory; positive regulation of peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; cell differentiation; response to electrical stimulus; phosphorylation; neuromuscular junction development