Human CC Chemokine Receptor 4 ELISA Kit | CCR4 elisa kit

Human CC Chemokine Receptor 4 ELISA Kit

Cat. Num. AAA744357

• Gene Names: CDK6; PLSTIRE
• Reactivity: Human
• Synonyms: CC Chemokine Receptor 4; Human CC Chemokine Receptor 4 ELISA Kit; CCR4 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500 pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This CDK6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDK6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR4 elisa kit

Principle of the Assay: CDK6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDK6 antibody and an CDK6-HRP conjugate. The assay sample and buffer are incubated together with CDK6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK6 concentration since CDK6 from samples and CDK6-HRP conjugate compete for the anti-CDK6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK6 from the sample, fewer sites are left to bind CDK6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCR4 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 223718134
• NCBI GeneID: 1021
• NCBI Accession #: NP_001138778.1
• NCBI GenBank Nucleotide #: NM_001145306.1
• Molecular Weight: 36,938 Da
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin-dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CDK6
• UniProt Synonym Gene Names: CDKN6
• UniProt Entry Name: CDK6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Uniprot Description

CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.

Protein type: Kinase, protein; Cell cycle regulation; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: nucleoplasm; ruffle; centrosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: Notch signaling pathway; dentate gyrus development; negative regulation of myeloid cell differentiation; response to virus; gliogenesis; T cell differentiation in the thymus; astrocyte development; protein amino acid phosphorylation; negative regulation of cell cycle; regulation of erythrocyte differentiation; negative regulation of cell proliferation; negative regulation of cell differentiation; positive regulation of fibroblast proliferation; regulation of gene expression; cell division; generation of neurons; cell dedifferentiation; negative regulation of osteoblast differentiation; mitotic cell cycle; positive regulation of cell-matrix adhesion; cell cycle arrest; lateral ventricle development; negative regulation of epithelial cell proliferation; G1/S transition of mitotic cell cycle

Disease: Microcephaly 12, Primary, Autosomal Recessive