Human CDK6 ELISA Kit | CDK6 elisa kit
Human CDK6 (Cyclin Dependent Kinase 6) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CDK6. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CDK6 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDK6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK6. You can calculate the concentration of Human CDK6 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
Uniprot Description
CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.
Protein type: Protein kinase, CMGC; Kinase, protein; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily
Chromosomal Location of Human Ortholog: 7q21-q22
Cellular Component: nucleoplasm; ruffle; centrosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleus
Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; ATP binding
Biological Process: Notch signaling pathway; dentate gyrus development; negative regulation of myeloid cell differentiation; response to virus; gliogenesis; T cell differentiation in the thymus; protein amino acid phosphorylation; astrocyte development; negative regulation of cell cycle; regulation of erythrocyte differentiation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; negative regulation of cell differentiation; cell division; regulation of gene expression; generation of neurons; cell dedifferentiation; negative regulation of osteoblast differentiation; mitotic cell cycle; positive regulation of cell-matrix adhesion; cell cycle arrest; lateral ventricle development; G1/S transition of mitotic cell cycle; negative regulation of epithelial cell proliferation
Disease: Microcephaly 12, Primary, Autosomal Recessive
