Canine B-Cell Activating Factor Receptor ELISA Kit | BAFFR elisa kit

Canine B-Cell Activating Factor Receptor ELISA Kit

Cat. Num. AAA743414

• Gene Names: TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• Reactivity: Canine
• Synonyms: B-Cell Activating Factor Receptor; Canine B-Cell Activating Factor Receptor ELISA Kit; BAFFR elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This BAFFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BAFFR. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAFFR elisa kit

Principle of the Assay: BAFFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BAFFR antibody and an BAFFR-HRP conjugate. The assay sample and buffer are incubated together with BAFFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAFFR concentration since BAFFR from samples and BAFFR-HRP conjugate compete for the anti-BAFFR antibody binding site. Since the number of sites is limited, as more sites are occupied by BAFFR from the sample, fewer sites are left to bind BAFFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAFFR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BAFFR elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 85567349
• NCBI GeneID: 115650
• Molecular Weight: 18,935 Da
• NCBI Official Full Name: BAFF receptor
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 13C
• NCBI Official Symbol: TNFRSF13C
• NCBI Official Synonym Symbols: BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 13C; BAFF receptor; BLyS receptor 3; B cell-activating factor receptor; B-cell-activating factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 13C
• UniProt Gene Name: TNFRSF13C
• UniProt Synonym Gene Names: BAFFR; BR3; BAFF-R
• UniProt Entry Name: TR13C_HUMAN

NCBI Description

B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. [provided by RefSeq, Jul 2008]

Uniprot Description

BAFF-R: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4); also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell cycle regulation; Cell surface; Receptor, misc.

Chromosomal Location of Human Ortholog: 22q13.1-q13.31

Cellular Component: integral to membrane; external side of plasma membrane

Biological Process: B cell costimulation; B cell homeostasis; T cell costimulation; positive regulation of germinal center formation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 4