Typical Testing Data/Standard Curve (for reference only)
Canine Integrin alpha2beta1 ELISA Kit | IG-alpha2beta1 elisa kit
Canine Integrin alpha2beta1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This IG-?2?1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IG-?2?1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY IG-?2?1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IG-?2?1 antibody and an IG-?2?1-HRP conjugate. The assay sample and buffer are incubated together with IG-?2?1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IG-?2?1 concentration since IG-?2?1 from samples and IG-?2?1-HRP conjugate compete for the anti-IG-?2?1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IG-?2?1 from the sample, fewer sites are left to bind IG-?2?1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IG-?2?1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: focal adhesion; cell surface; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane
Molecular Function: collagen binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; metal ion binding; laminin binding
Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; response to muscle activity; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; positive regulation of leukocyte migration; cell-matrix adhesion; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of collagen binding; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to organic cyclic substance; response to L-ascorbic acid; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; integrin-mediated signaling pathway; substrate-bound cell migration; response to drug; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; cell proliferation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; positive regulation of phagocytosis, engulfment; blood coagulation; response to amine stimulus; positive regulation of transmission of nerve impulse; positive regulation of inflammatory response
Disease: Bleeding Disorder, Platelet-type, 9
Research Articles on IG-alpha2beta1
Similar Products
Product Notes
The Canine IG-alpha2beta1 itga2 (Catalog #AAA741845) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741845 ELISA Kit recognizes Canine IG-alpha2beta1. It is sometimes possible for the material contained within the vial of "Integrin alpha2beta1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
