Porcine Integrin alpha2beta1 ELISA Kit | IG-alpha2beta1 elisa kit

Porcine Integrin alpha2beta1 ELISA Kit

Cat. Num. AAA737114

• Gene Names: ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• Reactivity: Porcine
• Synonyms: Integrin alpha2beta1; Porcine Integrin alpha2beta1 ELISA Kit; Porcine Integrin a2b1 ELISA Kit; IG-alpha2beta1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This IG-?2?1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IG-?2?1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IG-alpha2beta1 elisa kit

Principle of the assay: IG-?2?1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IG-?2?1 antibody and an IG-?2?1-HRP conjugate. The assay sample and buffer are incubated together with IG-?2?1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IG-?2?1 concentration since IG-?2?1 from samples and IG-?2?1-HRP conjugate compete for the anti-IG-?2?1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IG-?2?1 from the sample, fewer sites are left to bind IG-?2?1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IG-?2?1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IG-alpha2beta1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 116295258
• NCBI GeneID: 3673
• NCBI Accession #: NP_002194.2
• NCBI GenBank Nucleotide #: NM_002203.3
• Molecular Weight: 129,295 Da
• NCBI Official Full Name: integrin alpha-2
• NCBI Official Synonym Full Names: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• NCBI Official Symbol: ITGA2
• NCBI Official Synonym Symbols: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• NCBI Protein Information: integrin alpha-2; collagen receptor; platelet antigen Br; platelet glycoprotein GPIa; VLA2 receptor, alpha-2 subunit; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; human platelet alloantigen system 5; very late activation protein 2 receptor, alpha-2 subunit
• UniProt Protein Name: Integrin alpha-2
• UniProt Gene Name: ITGA2
• UniProt Synonym Gene Names: CD49B; GPIa
• UniProt Entry Name: ITA2_HUMAN

NCBI Description

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cell surface; focal adhesion; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane

Molecular Function: viral receptor activity; collagen binding; integrin binding; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; response to muscle activity; extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; positive regulation of smooth muscle cell proliferation; cell-matrix adhesion; positive regulation of leukocyte migration; positive regulation of collagen biosynthetic process; positive regulation of collagen binding; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to organic cyclic substance; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; response to drug; integrin-mediated signaling pathway; substrate-bound cell migration; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; cell proliferation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; blood coagulation; positive regulation of phagocytosis, engulfment; response to amine stimulus; positive regulation of inflammatory response; positive regulation of transmission of nerve impulse

Disease: Bleeding Disorder, Platelet-type, 9