Chicken Proteinase 3 antibody ELISA Kit | PR3 elisa kit

Chicken Proteinase 3 antibody ELISA Kit

Cat. Num. AAA739207

• Reactivity: Chicken
• Synonyms: Proteinase 3 antibody; Chicken Proteinase 3 antibody ELISA Kit; PR3 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PR3AB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PR3AB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PR3 elisa kit

Principle of the Assay: PR3AB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Proteinase 3 antigen (PR3AB) . Standards or samples are then added to the microtiter plate wells and PR3AB if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of PR3AB present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated Proteinase 3 antigen (PR3AB) are added to each well to "sandwich" the PR3AB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PR3AB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PR3AB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PR3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1335280
• UniProt Accession #: P24158; P15637; P18078; Q4VB08; Q4VB09; Q6LBM7; Q6LBN2; Q9UD25; Q9UQD8
• Molecular Weight: 27,807 Da
• NCBI Official Full Name: proteinase 3
• UniProt Protein Name: Myeloblastin
• UniProt Gene Name: PRTN3
• UniProt Synonym Gene Names: MBN; PR-3; PR3; NP-4
• UniProt Entry Name: PRTN3_HUMAN

Uniprot Description

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; EC 3.4.21.76; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol

Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: collagen catabolic process; negative regulation of phagocytosis; positive regulation of cell proliferation; proteolysis; blood coagulation