Rat PR3 (Proteinase 3) CLIA Kit | PR3 clia kit
Rat PR3 (Proteinase 3) CLIA Kit
Gene Names
PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
Reactivity
Rat
Synonyms
PR3 (Proteinase 3); Rat PR3 (Proteinase 3) CLIA Kit; PR3 clia kit
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant PR3. No significant cross-reactivity or interference between PR3 and analogues was observed.
Sequence Length
254
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PR3 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of PR3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PR3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PR3. You can calculate the concentration of PR3 in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PR3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PR3. You can calculate the concentration of PR3 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,807 Da
NCBI Official Full Name
proteinase 3
NCBI Official Synonym Full Names
proteinase 3
NCBI Official Symbol
PRTN3
NCBI Official Synonym Symbols
MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
NCBI Protein Information
myeloblastin
UniProt Protein Name
Myeloblastin
UniProt Gene Name
PRTN3
UniProt Synonym Gene Names
MBN; PR-3; PR3; NP-4
UniProt Entry Name
PRTN3_HUMAN
Uniprot Description
PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.76; Cell cycle regulation; Protease
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol
Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: negative regulation of phagocytosis; collagen catabolic process; positive regulation of cell proliferation; blood coagulation; proteolysis
