Goat RAC2 Polyclonal Antibody | anti-RAC2 antibody

Goat anti-RAC2 Antibody

Cat. Num. AAA420906

• Gene Names: RAC2; Gx; EN-7; HSPC022; p21-Rac2
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RAC2; Polyclonal Antibody; Goat anti-RAC2 Antibody; ras-related C3 botulinum toxin substrate 2 (rho family; small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family; small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family; small GTP-; RAC2 antibody; small GTP binding protein Rac2) antibody; EN-7 antibody; Ras-related C3 botulinum toxin substrate 2 antibody; small GTP binding protein Rac2 antibody; small GTP-binding protein Rac2) antibody; Gx antibody; HSPC022 antibody; small G protein antibody; anti-RAC2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PQPTRQQKRACSLL
• Sequence Length: 192

• Applicable Applications for anti-RAC2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative. Recommended concentration 0.3ug/ml. Data were obtained from a previous batch.; Western Blot: Approx 22kDa band observed in lysates of Human Thymus and Tonsil and of cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Thymus (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.3ug/ml) staining of MOLT4 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506381
• NCBI GeneID: 5880
• NCBI Accession #: NP_002863.1
• NCBI GenBank Nucleotide #: NM_002872.4
• Molecular Weight: 21,429 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 2
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• NCBI Official Symbol: RAC2
• NCBI Official Synonym Symbols: Gx; EN-7; HSPC022; p21-Rac2
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 2
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 2
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC2
• UniProt Entry Name: RAC2_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]

Uniprot Description

RAC2: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Interacts with DOCK2, which may activate it. Hematopoietic specific. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: actin filament; cytosol; focal adhesion; lamellipodium; nuclear envelope; phagocytic vesicle membrane; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein kinase regulator activity

Biological Process: actin filament organization; bone resorption; cell projection biogenesis; chemotaxis; G-protein coupled receptor protein signaling pathway; intracellular protein transport; metabolic process; nucleocytoplasmic transport; platelet activation; positive regulation of cell proliferation; regulation of hydrogen peroxide metabolic process; regulation of mast cell degranulation; regulation of protein kinase activity; regulation of small GTPase mediated signal transduction; regulation of T cell proliferation; signal transduction; small GTPase mediated signal transduction

Disease: Neutrophil Immunodeficiency Syndrome