Typical Testing Data/Standard Curve (for reference only)
Mouse heart fatty acid binding protein, h-FABP ELISA Kit | H-FABP elisa kit
Mouse heart fatty acid binding protein, h-FABP ELISA Kit
Gene Names
FABP3; MDGI; FABP11; H-FABP; O-FABP
Reactivity
Mouse
Synonyms
heart fatty acid binding protein; h-FABP; Mouse heart fatty acid binding protein; h-FABP ELISA Kit; Mouse heart fatty acid binding protein ELISA Kit; h-FABP ELISA Kit (Mouse); H-FABP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of HFABP. No significant cross-reactivity or interference between HFABP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HFABP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for H-FABP elisa kit
Intended Uses: This HFABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HFABP. This ELISA kit for research use only!
Principle of the Assay: HFABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HFABP antibody and an HFABP-HRP conjugate. The assay sample and buffer are incubated together with HFABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HFABP concentration since HFABP from samples and HFABP-HRP conjugate compete for the anti-HFABP antibody binding site. Since the number of sites is limited, as more sites are occupied by HFABP from the sample, fewer sites are left to bind HFABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HFABP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HFABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HFABP antibody and an HFABP-HRP conjugate. The assay sample and buffer are incubated together with HFABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HFABP concentration since HFABP from samples and HFABP-HRP conjugate compete for the anti-HFABP antibody binding site. Since the number of sites is limited, as more sites are occupied by HFABP from the sample, fewer sites are left to bind HFABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HFABP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for H-FABP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,858 Da
NCBI Official Full Name
heart fatty acid binding protein
NCBI Official Synonym Full Names
fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
NCBI Official Symbol
FABP3
NCBI Official Synonym Symbols
MDGI; FABP11; H-FABP; O-FABP
NCBI Protein Information
fatty acid-binding protein, heart; M-FABP; OTTHUMP00000003898; fatty acid binding protein 11; mammary-derived growth inhibitor; muscle fatty acid-binding protein; Fatty acid-binding protein 3, muscle; heart-type fatty acid-binding protein
UniProt Protein Name
Fatty acid-binding protein, heart
UniProt Gene Name
FABP3
UniProt Synonym Gene Names
FABP11; MDGI
UniProt Entry Name
FABPH_HUMAN
NCBI Description
The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq]
Uniprot Description
Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Subcellular location: Cytoplasm.
Domain: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Research Articles on H-FABP
Similar Products
Product Notes
The Mouse H-FABP fabp3 (Catalog #AAA725708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725708 ELISA Kit recognizes Mouse H-FABP. It is sometimes possible for the material contained within the vial of "heart fatty acid binding protein, h-FABP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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