Related Product Information for NT-4 elisa kit
Principle of the Assay: NT-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NT-4. Standards or samples are then added to the microtiter plate wells and NT-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NT-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for NT-4 are added to each well to "sandwich" the NT-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NT-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT-4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for NT-4 elisa kit
NCBI Description
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]
Uniprot Description
NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.
Protein type: Cytokine; Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region
Molecular Function: neurotrophin p75 receptor binding; growth factor activity
Biological Process: epidermis development; mechanoreceptor differentiation; cell-cell signaling; long-term memory; regulation of neuron differentiation; adult locomotory behavior; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Glaucoma 1, Open Angle, O
Product Notes
The Rat NT-4 ntf4 (Catalog #
AAA723822) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723822 ELISA Kit recognizes Rat NT-4.
It is sometimes possible for the material contained within the vial of
"Neurotrophin-4, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
cannot be held
responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
details on this or any of our
other policies, please see our
Terms & Conditions page.
