Typical Testing Data/Standard Curve (for reference only)
Human Caveolin-1 ELISA Kit | Cav-1 elisa kit
Human Caveolin-1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT1 antibody and an CAT1-HRP conjugate. The assay sample and buffer are incubated together with CAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT1 concentration since CAT1 from samples and CAT1-HRP conjugate compete for the anti-CAT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT1 from the sample, fewer sites are left to bind CAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
Function: Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Ref.7
Subunit structure: Homooligomer
By similarity. Interacts (via the N-terminus) with DPP4; the interaction is direct
By similarity. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Interacts with BMX, BTK, CTNNB1, CDH1, GLIPR2, JUP, NOSTRIN, SNAP25 and STX1A
By similarity. Interacts with PACSIN2. Ref.11
Subcellular location: Golgi apparatus membrane; Peripheral membrane protein
By similarity. Cell membrane; Peripheral membrane protein
By similarity. Membrane › caveola; Peripheral membrane protein. Membrane raft
By similarity. Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae
By similarity. Ref.11
Post-translational modification: The N-terminus of both isoforms are blocked.Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. Ref.8 Ref.9
Sequence similarities: Belongs to the caveolin family.
Research Articles on Cav-1
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Product Notes
The Human Cav-1 cav1 (Catalog #AAA722563) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722563 ELISA Kit recognizes Human Cav-1. It is sometimes possible for the material contained within the vial of "Caveolin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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