Mouse Ras-related protein Rab-10 (RAB10) ELISA Kit | RAB10 elisa kit

Mouse Ras-related protein Rab-10 (RAB10) ELISA Kit

Cat. Num. AAA7204709

• Reactivity: Mouse
• Synonyms: Ras-related protein Rab-10 (RAB10); Mouse Ras-related protein Rab-10 (RAB10) ELISA Kit; RAB10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAB10. No significant cross-reactivity or interference between RAB10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RAB10 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAB10 elisa kit

Intended Uses: This RAB10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseRAB10. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: RAB10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RAB10 antibody and an RAB10-HRP conjugate. The assay sample and buffer are incubated together with RAB10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAB10 concentration since RAB10 from samples and RAB10-HRP conjugate compete for the anti-RAB10 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAB10 from the sample, fewer sites are left to bind RAB10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAB10 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RAB10 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 256222019
• NCBI GeneID: 10890
• NCBI Accession #: NP_057215.3
• NCBI GenBank Nucleotide #: NM_016131.4
• Molecular Weight: 22,541 Da
• NCBI Official Full Name: ras-related protein Rab-10
• NCBI Official Synonym Full Names: RAB10, member RAS oncogene family
• NCBI Official Symbol: RAB10
• NCBI Protein Information: ras-related protein Rab-10; GTP-binding protein RAB10; ras-related GTP-binding protein
• UniProt Protein Name: Ras-related protein Rab-10
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB10
• UniProt Entry Name: RAB10_HUMAN

NCBI Description

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]

Uniprot Description

RAB10: a protein of the GTPase superfamily, Rab family. May be involved in vesicular trafficking and neurotransmitter release. Localized to the inner face of the cell membrane by a lipid-anchor.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; phagocytic vesicle membrane; focal adhesion; cytoplasmic vesicle membrane; recycling endosome membrane; exocyst; plasma membrane; endosome membrane; trans-Golgi network; endosome

Molecular Function: GTPase activity; GDP binding; GTP binding; myosin V binding

Biological Process: metabolic process; establishment of neuroblast polarity; protein secretion; endosome transport; vesicle-mediated transport; regulation of exocytosis; antigen processing and presentation; Golgi to plasma membrane transport; intracellular protein transport; polarized epithelial cell differentiation; axonogenesis; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; Rab protein signal transduction; vesicle docking during exocytosis