Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal testis-specific protein 1 (PXT1) Standard Curve.)
Mouse Peroxisomal Testis-Specific Protein 1 (PXT1) ELISA Kit | PXT1 elisa kit
Mouse Peroxisomal Testis-Specific Protein 1 (PXT1) ELISA Kit
Gene Names
Pxt1; Stepp; 1700001G18Rik
Reactivity
Mouse
Synonyms
Peroxisomal Testis-Specific Protein 1 (PXT1); Mouse Peroxisomal Testis-Specific Protein 1 (PXT1) ELISA Kit; PXT1; MGC129569; STEPP; small testis-specific peroxisomal protein; PXT1 elisa kit
Reactivity
Mouse
Specificity
Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PXT1. No significant cross-reactivity or interference between Mouse PXT1 and analogues was observed.
Sequence Length
51
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal testis-specific protein 1 (PXT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal testis-specific protein 1 (PXT1) Standard Curve.)
Related Product Information for PXT1 elisa kit
Description: This Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PXT1 in samples. An antibody specific for PXT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PXT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL)-motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. The peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.
Background: Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL)-motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. The peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxisomal testis-specific protein 1
NCBI Official Synonym Full Names
peroxisomal, testis specific 1
NCBI Official Symbol
Pxt1
NCBI Official Synonym Symbols
Stepp; 1700001G18Rik
NCBI Protein Information
peroxisomal testis-specific protein 1
UniProt Protein Name
Peroxisomal testis-specific protein 1
Protein Family
UniProt Gene Name
Pxt1
UniProt Synonym Gene Names
Stepp
UniProt Entry Name
PXT1_MOUSE
Research Articles on PXT1
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Product Notes
The Mouse PXT1 pxt1 (Catalog #AAA9717205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717205 ELISA Kit recognizes Mouse PXT1. It is sometimes possible for the material contained within the vial of "Peroxisomal Testis-Specific Protein 1 (PXT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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