Rabbit anti-Human PITX1 Polyclonal Antibody | anti-PITX1 antibody

PITX1, ID (Pituitary Homeobox 1, Paired-like Homeodomain Transcription Factor 1, Homeobox Protein PITX1, Hindlimb-expressed Homeobox Protein Backfoot, PTX1, BFT) (MaxLight 650)

Cat. Num. AAA6496075

• Gene Names: PITX1; BFT; CCF; POTX; PTX1; LBNBG
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PITX1; Polyclonal Antibody; ID (Pituitary Homeobox 1; Paired-like Homeodomain Transcription Factor 1; Homeobox Protein PITX1; Hindlimb-expressed Homeobox Protein Backfoot; PTX1; BFT) (MaxLight 650); anti-PITX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PITX1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-PITX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PITX1 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PITX1 Antibody (Center) (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.)

Product Categories/Family for anti-PITX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Grimminger, P., Ling, F.C. Anticancer Res. 29(4), 1281-1286 (2009). 2. Gurnett, C.A., Alaee, F. Am. J. Hum. Genet. 83(5), 616-622 (2008). 3. Philippi, A., Tores, F. BMC Med. Genet. 8, 74 (2007). 4. Shang, J., Li, X. Genomics 40(1), 108-113 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 152963644
• NCBI GeneID: 5307
• NCBI Accession #: NP_002644
• NCBI GenBank Nucleotide #: NM_002653
• UniProt Accession #: P78337
• Molecular Weight: 34kDa
• NCBI Official Full Name: pituitary homeobox 1
• NCBI Official Synonym Full Names: paired like homeodomain 1
• NCBI Official Symbol: PITX1
• NCBI Official Synonym Symbols: BFT; CCF; POTX; PTX1; LBNBG
• NCBI Protein Information: pituitary homeobox 1
• UniProt Protein Name: Pituitary homeobox 1
• Protein Family: Pituitary homeobox
• UniProt Gene Name: PITX1
• UniProt Synonym Gene Names: BFT; PTX1
• UniProt Entry Name: PITX1_HUMAN

NCBI Description

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008]

Uniprot Description

PITX1: May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Defects in PITX1 are a cause of congenital clubfoot (CCF); also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus

Biological Process: myoblast cell fate commitment; anatomical structure morphogenesis; transcription, DNA-dependent; pituitary gland development; cartilage development; branchiomeric skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; embryonic hindlimb morphogenesis

Disease: Liebenberg Syndrome; Clubfoot, Congenital, With Or Without Deficiency Of Long Bones And/or Mirror-image Polydactyly