Rabbit alpha-2-macroglobulin Polyclonal Antibody | anti-A2M antibody
Rabbit anti-human alpha-2-macroglobulin polyclonal Antibody
WB : 1:2000-1:20000
IP : 1:200-1:1000
IHC : 1:20-1:200 (Recommender dilutions)
Positive WB detected in : Human blood tissue, HepG2 cells, human brain tissue, human serum tissue
Positive IP detected in: HepG2 cells Positive IHC detected in: Human hepatocirrhosis tissue, human ovary tissue, human stomach tissue
NCBI and Uniprot Product Information
NCBI Description
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
Uniprot Description
A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: extracellular region; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding
Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway
Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
Research Articles on A2M
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Product Notes
The A2M a2m (Catalog #AAA716966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human alpha-2-macroglobulin polyclonal Antibody reacts with Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-2-macroglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: Use at an assay dependent dilution. WB : 1:2000-1:20000 IP : 1:200-1:1000 IHC : 1:20-1:200 (Recommender dilutions) Positive WB detected in : Human blood tissue, HepG2 cells, human brain tissue, human serum tissue Positive IP detected in: HepG2 cells Positive IHC detected in: Human hepatocirrhosis tissue, human ovary tissue, human stomach tissue. Researchers should empirically determine the suitability of the A2M a2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-2-macroglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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