Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate All lanes: COMT Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 25 kDa Observed band size: 31 kDa)
COMT recombinant antibody
COMT Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate All lanes: COMT Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 25 kDa Observed band size: 31 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate All lanes: COMT Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 25 kDa Observed band size: 31 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
Uniprot Description
COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Methyltransferase; Membrane protein, integral; EC 2.1.1.6; Amino Acid Metabolism - tyrosine
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: postsynaptic membrane; mitochondrion; membrane; axon; plasma membrane; integral to membrane; dendritic spine; cytosol
Molecular Function: protein binding; magnesium ion binding; O-methyltransferase activity; catechol O-methyltransferase activity
Biological Process: response to drug; methylation; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to pain; response to lipopolysaccharide; learning; female pregnancy; reproductive process in a multicellular organism; response to organic cyclic substance; positive regulation of homocysteine metabolic process; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process
Disease: Schizophrenia; Panic Disorder 1
Research Articles on COMT
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Product Notes
The COMT comt (Catalog #AAA7136777) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COMT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the COMT comt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMT, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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