Rabbit anti-Human, Mouse CPB2 Polyclonal Antibody | anti-CPB2 antibody

CPB2 Antibody

Cat. Num. AAA7051873

• Gene Names: CPB2; CPU; PCPB; TAFI
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: CPB2; Polyclonal Antibody; CPB2 Antibody; Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI; anti-CPB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 386

• Applicable Applications for anti-CPB2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Carboxypeptidase B2 protein (225-372AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse liver tissueAll lanes: CPB2 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49,41 KDaObserved band size: 49 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7051873 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of PC3 cells using MBS7051873 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-CPB2 antibody

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

NCBI and Uniprot Product Information

• NCBI GI #: 513126877
• NCBI GeneID: 1361
• NCBI Accession #: NP_001265470.1
• NCBI GenBank Nucleotide #: NM_001278541.1
• UniProt Accession #: Q96IY4; Q15114; Q5T9K1; Q5T9K2; Q9P2Y6; A8K464
• Molecular Weight: 40,922 Da
• NCBI Official Full Name: carboxypeptidase B2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI

NCBI Description

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.20; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q14.13

Biological Process: fibrinolysis