Western Blot (WB)
(Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: METTL14 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)
Rabbit anti-Human METTL14 Polyclonal Antibody | anti-METTL14 antibody
METTL14 Antibody
Gene Names
METTL14; hMETTL14
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
METTL14; Polyclonal Antibody; METTL14 Antibody; N6-adenosine-methyltransferase subunit METTL14; Methyltransferase-like protein 14; KIAA1627; anti-METTL14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
456
Applicable Applications for anti-METTL14 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IHC: 1: 20-1: 200
Immunogen
Recombinant human N6-adenosine-methyltransferase subunit METTL14 protein (24-206AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: METTL14 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: METTL14 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7051412 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7051412 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7051412 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7051412 at dilution of 1:100)
Related Product Information for anti-METTL14 antibody
N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed: 24316715, PubMed: 24407421, PubMed: 25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,150 Da
NCBI Official Full Name
N6-adenosine-methyltransferase subunit METTL14
NCBI Official Synonym Full Names
methyltransferase like 14
NCBI Official Symbol
METTL14
NCBI Official Synonym Symbols
hMETTL14
NCBI Protein Information
N6-adenosine-methyltransferase subunit METTL14
UniProt Protein Name
N6-adenosine-methyltransferase subunit METTL14
Protein Family
UniProt Gene Name
METTL14
UniProt Synonym Gene Names
hMETTL14
UniProt Entry Name
MET14_HUMAN
Uniprot Description
METTL14: Probable methyltransferase. Belongs to the MT-A70-like family.
Protein type: EC 2.1.1.62; Methyltransferase
Chromosomal Location of Human Ortholog: 4q26
Cellular Component: nucleoplasm; nucleus
Molecular Function: mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity; mRNA binding; protein binding
Biological Process: gene expression; nuclear mRNA splicing, via spliceosome; stem cell maintenance
Research Articles on METTL14
Similar Products
Product Notes
The METTL14 mettl14 (Catalog #AAA7051412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METTL14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1: 1000-1: 5000 IHC: 1: 20-1: 200. Researchers should empirically determine the suitability of the METTL14 mettl14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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