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Western Blot (WB) (Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: PEMT antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23,26,25 kDaObserved band size: 23 kDa)

Rabbit anti-Human PEMT Polyclonal Antibody | anti-PEMT antibody

PEMT Antibody

Gene Names
PEMT; PLMT; PNMT; PEAMT; PEMPT; PEMT2
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
PEMT; Polyclonal Antibody; PEMT Antibody; Phosphatidylethanolamine N-methyltransferase (PEAMT) (PEMT) (EC 2.1.1.17) (EC 2.1.1.71) (PEMT2) (Phospholipid methyltransferase) (PLMT; PEMPT; PNMT; anti-PEMT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
214
Applicable Applications for anti-PEMT antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IF: 1:50-1:200
Immunogen
Recombinant Human Phosphatidylethanolamine N-methyltransferase protein (2-49AA)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Names
PEMT
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-130842
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: PEMT antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23,26,25 kDaObserved band size: 23 kDa)

Western Blot (WB) (Western BlotPositive WB detected in:SH-SY5Y whole cell lysateAll lanes: PEMT antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23,26,25 kDaObserved band size: 23 kDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7048851 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7048851 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-PEMT antibody
Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylethanolamine N-methyltransferase isoform 4
NCBI Official Synonym Full Names
phosphatidylethanolamine N-methyltransferase
NCBI Official Symbol
PEMT
NCBI Official Synonym Symbols
PLMT; PNMT; PEAMT; PEMPT; PEMT2
NCBI Protein Information
phosphatidylethanolamine N-methyltransferase
UniProt Protein Name
Phosphatidylethanolamine N-methyltransferase
UniProt Gene Name
PEMT
UniProt Synonym Gene Names
PEAMT; PEMT; PLMT
UniProt Entry Name
PEMT_HUMAN

NCBI Description

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

Uniprot Description

Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).

Research Articles on PEMT

Similar Products

Product Notes

The PEMT pemt (Catalog #AAA7048851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEMT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:1000-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the PEMT pemt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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